VICs were extracted from 6 healthy 6-monthold fresh porcine hearts by serial collagenase digestion. Quantitative polymerase chain reaction (qPCR) was used to quantify transdifferentiation of genes of interest during spontaneous calcification of VICs. Spontaneous calcification of VICs was increased by adding Na3PO4 (3 mM, pH 7.4).
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Submission link of articles to International Journal of Cardiovascular Research