To study cytomorphological changes in various ocular surface disorders and find out the sensitivity, specificity and the diagnostic accuracy of impression cytology. One hundred and ten patients (84 cases and 26 controls) were included in this study. Out of these 84 cases, 25 cases having vitamin A deficiency (VAD), 19 cases are having vernal keratoconjunctivitis (VKC), 30 of dry eye and 10 of chemical burn. Impression cytology was performed with each patient and the results were noted. Biopsy was done in 2 patients from each group. Patients were examined for goblet cell density in the form of clumping of goblet cells and degree of squamous metaplasia in terms of loss of cell cohesion, altered cell morphology and keratinisation.
Impression cytology was found to be good technique for diagnosing metaplastic changes in various ocular surface disorders.