Tissue Engineering (TE) aims to bio manufacture human tissues, an endeavor that requires a large number of cells, preferably autologous ones. Typically, an autologous cell source is unable to provide the required amount of cells and it is necessary that such cells be cultured during a prolonged period of time. One of the methodologies used to obtain and expand autologous cells in culture is explants; however, explants are commonly discarded at the moment of the first cell passage. Given the difficulty in obtaining those cell sources, a good alternative would be to reuse the explants and put them back into culture after cell passage. Nevertheless, various modifications have been seen in long-term cell culture, such as modifications of gene expression, and number and amount of proteins.
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